Branch migration inhibition in PCR-amplified DNA: homogeneous mutation detection

Screening for single-nucleotide polymorphisms using branch migration inhibition in PCR-amplified DNA.

BACKGROUND New methods are required for the exploration of the human genome by discovering sequence variations. This study evaluated the performance of a new method for screening a large number of samples for several DNA polymorphisms. METHODS We used a homogeneous method based on inhibition of spontaneous branch migration by any sequence difference between two molecules of PCR-amplified DNA....

Amplified migration inhibition effect.

When tuberculin-sensitive peritoneal exudate cells are incubated in a culture flask with tuberculin purified protein derivative, macrophage inhibition factor and other lymphokines are released into the culture medium. We have described how, if incubation is carried out in a stationary conical culture tube, intercellular contact between the peritoneal exudate cells is facilitated as the cells se...

Sequencing of PCR-amplified DNA.

Alternatives for sequencing of PCR products essentially fall into one of two categories; generation of single-stranded DNA for sequencing or the direct sequencing of double-stranded product. Of the two alternatives, sequencing of double-stranded PCR products is likely to be of greatest immediate significance in terms of general applicability and rapidity. Double-stranded sequencing allows the u...

Molecular Identification of PCR and TaqMan Automated Detection in Utility of Random Amplified Polymorphic DNA

Label-Free Colorimetric Detection of Specific Sequences in PCR amplified DNA

We observed that single-stranded DNA adsorbs on negatively charged gold nanoparticles (Au-nps) and the adsorption rate is sequence length dependent. We also found that the adsorption stabilizes the Au-nps against salt-induced aggregation. Based on these observations, we developed a simple, rapid and cost efficient colorimetric assay to identify specific sequences in PCR amplified DNA. The assay...